Simultaneous acquisition of diffusion tensor imaging (DTI) and magnetic resonance elastography (MRE) has been proven feasible in previous pre-clinical studies. DTI-MRE requires the identification of experimental parameter ranges that depend on the specific target. Here we identify valid experimental parameter sets in simulations for in vivo human brain applications and the DTI-MRE technique is implemented by modifying a single-shot spin-echo EPI sequence. A good agreement was found between DTI-MRE and conventional acquisitions. DTI-MRE may increase the clinical acceptance of MRE and DTI by providing co-registered diffusion and tissue mechanical property maps within the scan time of a conventional DTI acquisition.