Resting state fMRI (rs-fMRI) can potentially be used to measure cerebrovascular reactivity (CVR). Specifically, resting state fluctuation amplitude (RSFA) is related to the breath hold response. In this study a multiband (MB), ME sequence was compared to a MB, single echo (SE) sequence in terms of their ability to estimate CVR using rs-fMRI-derived RSFA by comparing to BH-CVR. The MBME scan showed higher voxelwise across-subject correlation between RSFA and CVR. The spatial correlation between mean RSFA and CVR maps was also higher for MBME scans, indicating improved accuracy of RSFA as an alternative to CVR using an MBME sequence.