Orientation selective deep brain stimulation (OS-DBS) is a flexible strategy to achieve selective stimulation of brain pathways. OS-DBS was used here in rats for stimulating the entorhinal cortex (EC) and the medial septal nucleus (MSN). Stimulating such areas induce neurogenesis in dental gyrus of the hippocampal formation, and can thus be regarded a potential therapy for Alzheimer’s disease (AD). Brain responses during OS-DBS were monitored by MB-SWIFT fMRI at 9.4T. Results demonstrate that different orientations of OS-DBS can modulate and selectively maximize activation of EC/MSN- hippocampal formation networks, and thus hold promise for maximizing neurogenesis and therapy efficacy for AD.