Filter-Exchange Imaging (FEXI) is a noninvasive double-diffusion imaging method, sensitive to transmembrane water exchange, which is strongly connected to cell viability. A FEXI sequence was implemented and tested in vitro with baker’s yeast. Upon permeabilization with ethanol, AXR increased whereas ADC decreased, more so with increasing ethanol concentration. AXR reduced over time, but only minor changes in ADC, intracellular volume and Trypan staining were detected.
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