Focal cortical ischemia animal model has been widely used to study neuroanatomical reorganization of cortex with intracortical microstimulation. However, the whole-brain structural changes following focal ischemia on motor cortex is unknown. In this study, high-resolution DTI is performed on focal unilateral motor cortex ischemia rat model at 14T. Voxel-wise based analysis (VBA) and VBA-guided connectivity analysis are performed to explore potential whole-brain microstructure and global structural connectivity changes. Our results show that large-scale white matter microstructural changes mainly distribute in corpus callosum, external capsule, cerebral peduncle. Significant reorganization of sensorimotor network associated with these regions were also found after ischemia.