Three-dimensional Polarized Light Imaging (3D-PLI) is an optical technique presented as a good candidate for validation of diffusion Magnetic Resonance Imaging (dMRI) results such as orientation estimates and tractography. We previously demonstrated a multi-scale tractography approach using 3D-PLI. Here, we show how tractograms obtained at different spatial scales from 3D-PLI human brain datasets can be inspected using homology theory in order to perform a quantitative comparison between them. This paves the way for the validation of tractography obtained from dMRI using multi-scale fiber tracking based on 3D-PLI.
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