Here we show the first DTI phantom manufactured by advanced high-resolution 3D-printing methods. The phantom consists of hollow, 12μm thin, liquid filled channels, that can be constructed in arbitrary configurations, ideally suited for validating diffusion sequences and analysis models. A simple configuration with orthogonal channel directions is presented. Diffusion weighted images were acquired and a diffusion tensor model employed. The main direction of the resulting tensors is accurately able to capture the directions of the channels with an average fractional anisotropy of 0.46. This method for creating diffusion phantoms will help to test and validate different models in the future.