A spinal cord-mimicking fibre phantom was developed to evaluate its potential for validating dMRI. Microfibres were co-electrospun with two polymer solutions and characterized by SEM. The phantom comprised two material samples designed with 0o and 90o crossings. SEM revealed that fibres were uniaxially aligned and hollow, having similar sizes to spinal cord axons. Diffusion tensor analysis of a ZOOM dMRI acquisition demonstrated the difference in alignment of the two samples. Diffusion kurtosis analysis demonstrated differences in axial and radial diffusion restriction, with parameter values consistent with published spinal cord data. Relaxation time constants were similar in two samples.