The labelled SPIO of the transplanted MSCs were found in spinal cord segment of TSCI based on T1w and T2w images. The DTI can be of great value in the dynamic evaluation of morphological and neurological changes in beagles after TSCI. FA values are correlated with the impairment and repair of the fiber tracts of the spinal cord. The FA value can satisfactorily evaluate the completeness of the fiber tracts after SCI and the repair of them by stem cells. The combination of conventional MRI and DTI is of great clinical importance for the diagnosis and therapeutic evaluation of SCI.