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Abstract #4712

Glymphatic monitoring in awake state mouse brain with DCE-MRI imaging

Niklas Daniel Åke Persson1, Evan Hunter Stanton 1, Björn Sigurdsson1, Tuomas Lilius1, Humberto Mestre2, Maiken Nedergaard1,2, and Yuki Mori1,3
1Division of Glial Disease and Therapeutics, University of Copenhagen, Center for Translational Neuromedicine, Copenhagen, Denmark, 2Division of Glial Disease and Therapeutics, University of Rochester Medical Center, Center for Translational Neuromedicine, Rochester, NY, United States, 3Faculty of Health and Medical Sciences, University of Copenhagen, Panum NMR Core Facility, Copenhagen, Denmark

The glymphatic pathway is a novel pathway for interstitial solute clearance. Previous reports note that glymphatic patterns differ according to brain activity. Due to the motion-induced image blurring effects, DCE-MRI in the awake animal is difficult. We aimed to determine the feasibility of FISP-based fast DCE-MRI to assess the glymphatic pathway in awake mice. Head-fixed mice were habituated to restraint and received a cisterna magna cannulation. Awake DCE-MRI was performed 24 hours post surgery. Dynamic tracer distribution maps were calculated, and tracer distribution was measured in each brain segmentation. We successfully obtain DCE-MRI and demonstrate glymphatic dynamics in awake mice.

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