The glymphatic pathway is a novel pathway for interstitial solute clearance. Previous reports note that glymphatic patterns differ according to brain activity. Due to the motion-induced image blurring effects, DCE-MRI in the awake animal is difficult. We aimed to determine the feasibility of FISP-based fast DCE-MRI to assess the glymphatic pathway in awake mice. Head-fixed mice were habituated to restraint and received a cisterna magna cannulation. Awake DCE-MRI was performed 24 hours post surgery. Dynamic tracer distribution maps were calculated, and tracer distribution was measured in each brain segmentation. We successfully obtain DCE-MRI and demonstrate glymphatic dynamics in awake mice.