With increased interests in understanding the hippocampal regulation of hypothalamic-pituitary-adrenocortical (HPA) axis, this study shows for the first time an in vivo comparison of metabolic fluxes in the mouse dorsal hippocampus and hypothalamus using indirect 1H-[13C] MRS upon [1,6-13C2] glucose infusion at 14.1T. This study provides foundation for investigating relevant evidence to the underlying mechanism of hippocampal regulation of HPA axis.