Abstract #0294

Exploring the epileptic rat hippocampus using oscillating gradients, 3D electron microscopy and Monte Carlo simulations

Jonathan Scharff Nielsen¹, Alejandra Sierra², Ilya Belevich³, Eija Jokitalo³, and Manisha Aggarwal¹

¹Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, United States, ²A.I. Virtanen Institute of Molecular Sciences, University of Eastern Finland, Kuopio, Finland, ³Institute of Biotechnology, University of Helsinki, Helsinki, Finland

Oscillating gradient spin-echo (OGSE) diffusion MRI (dMRI) is sensitive to small-scale restrictions and may provide a sensitive probe of gray matter microstructural changes in brain disorders such as temporal lobe epilepsy. However, relating the OGSE spectral changes to specific microstructural features is a difficult challenge. Here, we combined OGSE-dMRI with serial block-face electron microscopy volumes of healthy and status epilepticus exhibiting rat hippocampi. From tissue parameters extracted from these volumes, we generated 3D digital substrates for Monte-Carlo random-walk simulations, which allowed us to elucidate the relative contributions of underlying gray matter microstructural features to the OGSE measurements.

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