Viral vectors, including adeno-associated viruses (AAV), are increasingly used for therapies that utilize somatic cell gene editing. Validation of successful delivery of gene editing machinery requires biopsy, which is a fundamental barrier to development of gene therapy. Research into chemical exchange saturation transfer (CEST)-MRI contrast agents has yielded numerous targeted and pH sensitive exogenous agents for diagnostic purposes. In this study we demonstrated that CEST-MRI can detect the abundant serine, threonine, and lysine residues on AAV capsid surfaces across serotypes that are responsible for targeted receptor binding; further that transduction of cells by AAV2 can be detected by CEST.
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