Grey matter atrophy is a marker of progressive disability in multiple sclerosis (MS). To better study atrophy in MS, mouse models that display grey matter loss are needed. A possible candidate is the cuprizone mouse model, which exhibits demyelination, gliosis, and axonal injury. We used high-resolution MRI (37.5x37.5x250μm3) and atlas-based volumetrics to measure volumes of 62 structures in the brains of cuprizone mice following acute (6-weeks) and chronic (12-weeks) demyelination. We found no atrophy associated with acute demyelination but identified atrophy in 7 regions following chronic demyelination including the corpus callosum, internal capsule, striatum, and thalamus.