The impairment of microvessels can lead to neurologic diseases such as stroke and vascular dementia. The imaging of lumen and vessel wall of perforating arteries requires an extremely high resolution due to their small caliber size. In this study, we developed a 3D inner-volume (IV) TSE (SPACE) sequence with optimized spatially selective excitation (SSE) RF pulses. High resolution of isotropic 0.30mm within ten minutes was achieved for the black- blood images of lenticulostriate artery (LSA) for the first time. The IV-SPACE images showed clearer delineation of vessel wall and lumen of LSA than conventional SPACE images.