Abstract #0926

In-Vivo Cell Tracking of Murine Natural Killer Cells in Lymphoma by Fluorine-19 MRI

Lawrence Lechuga¹, Sean B Fain^1,2,3,4, Christian M Capitini^3,4,5, and Matthew H Forsberg⁵

¹Medical Physics, University of Wisconsin, Madison, Madison, WI, United States, ²Radiology, University of Wisconsin, Madison, Madison, WI, United States, ³Biomedical Engineering, University of Wisconsin, Madison, Madison, WI, United States, ⁴Carbone Cancer Center, University of Wisconsin, Madison, Madison, WI, United States, ⁵Pediatrics, University of Wisconsin, Madison, Madison, WI, United States

Fluorine-19 (¹⁹F) MRI can monitor various cell types in vivo for days to weeks after injection of 1⁹F-labeled cells. To establish cell viability Green Fluorescent Protein (GFP+) murine natural killer (NK) cells were labeled ex vivo using red-fluorescent perfluoropolyether (PFPE-red) and delivered intratumorally into 3 lymphoma-bearing mice. Flow cytometry validated that the infused NK cells retain their ¹⁹F label out to 6 days post-injection (PI). Cells were then tracked and quantified via ¹H/¹⁹F MRI out to 6 days PI. Quantification of the infused cells indicate 87% and 70% were detectible at days 0 and 6, respectively.

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