Relaxation Along a Fictitious Field (RAFF) times in the 2nd rotating frame (TRAFF2) have shown promise for the depiction of acute myocardial infarct in a mouse model. In this work, in vivo myocardial TRAFF2 maps are acquired in a single breath-hold at 3T. An additional image was acquired following saturation preparation to enable reconstruction with a three-parameter fit model. Visually high-quality map quality, homogeneous signal, artifact resilience and low inter-scan variability were achieved with TRAFF2 in vivo. This makes TRAFF2 an excellent candidate for myocardial tissue characterization in clinical use.