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Abstract #1223

Investigating differences in lung cancer cells with induced and de-induced cisplatin resistance by 1H HR-MAS NMR metabolomics

Martina Vermathen1, Hendrik von Tengg-Kobligk2,3, Martin Nils Hungerb├╝hler2,3, Christoph Kempf2,3, Peter Vermathen2,3, and Nico Ruprecht2,3
1Department of Chemistry and Biochemistry, University Bern, Bern, Switzerland, 2University Institute of Diagnostic, Interventional and Pediatric Radiology, University Bern, Bern, Switzerland, 3Department of BioMedical Research, University Bern, Bern, Switzerland

Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is a major problem. Here, a high resolution magic angle spinning (HR-MAS) NMR study is presented determining the metabolic profile of lung cancer cells (A24) and metabolic adaptations in different levels of induced cisPt-resistance as well as in their de-induced counterparts. More than 40 metabolites were identified. Metabolic adaptations were determined, which increased with higher cisPt-resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione or taurine.

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