Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is a major problem. Here, a high resolution magic angle spinning (HR-MAS) NMR study is presented determining the metabolic profile of lung cancer cells (A24) and metabolic adaptations in different levels of induced cisPt-resistance as well as in their de-induced counterparts. More than 40 metabolites were identified. Metabolic adaptations were determined, which increased with higher cisPt-resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione or taurine.