Temporal lobe epilepsy (TLE) is the most common type of epilepsy in adults. Language impairment can result from both continued seizures and surgical attempts to treat it. Thus, accurate preoperative assessment of language function is essential. Here we used resting-state fMRI to investigate the altered amplitude of low frequency fluctuation (ALFF) in language eloquent areas in medically-refractory TLE patients and its relationship with clinical language test measures. Our findings suggest that left TLE disrupts language function more than right TLE and that intrinsic spontaneous brain activity is altered even in the absence of detectable clinical language impairment.