Standardized blood tests of kidney function lack adequate sensitivity to capture chronic progression of renal injuries. We examined whether saline-responsive urea CEST and quantitative MT imaging can be used to detect mild renal injury development. Upon renal injury progression, the urea CEST contrast decreased in the cortex, and the saline-induced fold change of contrast decreased in the inner medulla and papilla. qMT imaging showed the decrease of semi-solid macromolecule pool in the cortex. These results indicate that the saline-responsive CEST and qMT imaging have a potential to detect subclinical renal injury development.