Clinical functional MRI (fMRI) can be limited by neurovascular uncoupling (NVU) resulted from the pathology. Cerebrovascular reactivity (CVR) mapping based on BOLD MRI during a hypercapnia task, such as breath hold (BH), has been proposed to indicate the potential NUV. However, BH-MRI is limited by patient’s corporation. Recent studies have suggested that CVR measurement can be derived from resting-state (rs) fMRI that has also been increasing utilized to map functional networks in clinical settings. This study evaluates the CVR mapping with rs-fMRI and compare with BH-MRI in patients with gliomas.