Abstract #3837

Characterization of the triglyceride fatty acid composition and adipocyte size in human adipose tissue using SHORTIE STEAM: in vitro validation

Stefan Ruschke¹, Julius Honecker², Dominik Weidlich¹, Claudine Seeliger², Olga Prokopchuk³, Josef Ecker⁴, Marcus R. Makowski¹, Hans Hauner², and Dimitrios C. Karampinos¹

¹Department of Diagnostic and Interventional Radiology, School of Medicine, Technical University of Munich, Munich, Germany, ²Else Kröner Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany, ³Department of Surgery, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, Munich, Munich, Germany, ⁴ZIEL Institute for Food & Health; Research Group Lipid Metabolism, Technical University of Munich, Freising, Germany

Short-TR multi-TI multi-TE (SHORTIE) STEAM is a promising technique for the characterization of adipose tissue since it allows the non-invasive simultaneous probing of triglyceride fatty acid composition and triglyceride T1- and T2-relaxation times. The present in vitro study of human adipose tissue aims at the validation of the SHORTIE-assessed triglyceride fatty acid composition with gas chromatography–mass spectrometry and investigates potential correlations of the triglyceride relaxation properties with adipocyte cell size from histology.

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