The precision (repeatability and reproducibility) of quantitative stimulated echo acquisition mode (STEAM) MRS was evaluated in subjects with known or suspected liver iron overload at both 1.5T and 3T. The test-retest repeatability and reproducibility across acquisition modes (multi-TE vs multi-TE-TR) of STEAM-MRS multi-parametric quantification was evaluated using linear regression and Bland-Altman analyses. STEAM-MRS demonstrated high precision for several biomarkers, including R2water and R2fat, FWHMwater and FWHMfat as surrogates for R2*, and PDFF, at both 1.5T and 3T. R1water and R1fat exhibited moderate repeatability. The precision of the water estimates (R1, R2, and FWHM) was relatively similar at both field strengths.