Abstract #0140

Mechanism of recovery following stem cell therapy in ischemic stroke: cell or secretome?

Shannon Helsper^1,2, Xuegang Yuan³, Richard Jeske², Li Sun⁴, David G. Meckes Jr.⁴, Yan Li², and Samuel C. Grant^1,2

¹National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, United States, ²Chemical & Biomedical Engineering, FAMU-FSU College of Engineering, Tallahassee, FL, United States, ³University of California, Los Angeles, CA, United States, ⁴Department of Biomedical Sciences, Florida State University, Tallahassee, FL, United States

Synopsis

This study interrogates human mesenchymal stem cell (hMSC) derived treatments (cells versus secretome) in a preclinical transient ischemic stroke model. Biochemical markers of tissue recovery measured longitudinally over 21 d via sodium chemical shift imaging and proton relaxation-enhanced MR spectroscopy demonstrated that the arterial injection of extracellular vesicles produced in vitro by hMSC improved outcomes over ischemic stroke, albeit not to the level of direct cellular implantation. For the first time, novel MRI-compatible labeling provided the initial visualization of biodistribution and estimated permanence of hMSC-derived extracellular vesicles in the ischemic brain.

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