Telomerase reverse transcriptase (TERT) expression is essential for tumor proliferation. TERT also reprograms metabolism by elevating NADH and upregulating the alanine transporter ASCT2. Here, we exploit these TERT-associated metabolic alterations for non-invasive imaging in live GBM cells and orthotopic tumors using hyperpolarized [1-13C]-alanine. Combined treatment with the TERT inhibitor 6-thio-2’-deoxyguanosine and the ASCT2 inhibitor V-9302 inhibits GBM proliferation, identifying a novel therapeutic opportunity. Importantly, lactate production from hyperpolarized [1-13C]-alanine is an early biomarker of response to treatment in vivo, prior to the onset of anatomical alterations. Our findings pave the way for improved therapy and response assessment for GBM patients.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords