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Abstract #0379

Magnetic resonance imaging of cationic compounds using their proton exchange modulating effect

Zheng Han1,2, Safiya Aafreen3, Yang Zhou1,2,4, Chongxue Bie1,2, Jiadi Xu1,2, Lei Zheng5, Peter C.M. van Zijl1,2, and Guanshu Liu1,2
1Radiology, Johns Hopkins University, Baltimore, MD, United States, 2F.M Kirby Research Center, Kennedy Krieger Institute, Baltimore, MD, United States, 3Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States, 4Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China, 5Oncology, Johns Hopkins University, Baltimore, MD, United States


Here we report a novel approach to detect non-labeled cationic compounds, named modulated saturation transfer (MOST)-MRI, based on their effect of modulating the exchange rate of surrounding exchangeable protons. MOST-MRI was able to detect polyethylenimine (PEI600, M.W. = 600 Da), at a high sensitivity (μM or μg/mL range) through its quenching effects on surrounding fast-exchanging protons. In animals, MOST-MRI detected the tumor uptake of PEI600 that was injected intratumorally at a low dose (50 μg/kg b.w.), indicating the potential of MOST-MRI for label-free imaging guidance that is needed for the development and clinical translation of cationic gene delivery systems.

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