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Abstract #0444

Correlation between pathophysiological functions of Glioma invasion and water dynamics by FFC-NMR in vivo

Maria Rosaria Ruggiero1, Hamza Aït Itto2, Simona Baroni1, Jean Boutonnat3, François Berger2, Lionel Marc Broche4, Silvio Aime1, Simonetta Geninatti1, and Hana Lahrech2
1University of Torino, Torino, Italy, 2BrainTech Lab INSERM U1205, Grenoble, France, 3CHU Grenoble, Grenoble, France, 4University of Aberdeen, Aberdeen, United Kingdom


R1-dispersion profiles of in vivo glioma mouse models acquired by Fast-Field-Cycling NMR (FFC-NMR) were found to discriminate invasion from proliferation at fields below 2mT. These differences were correlated to the transcytolemmal water-exchange, demonstrating the water cell influx/outflux role in relaxation mechanisms. Hypoxia and H2O2, two major pathophysiological processes of invasion, were demonstrated to modulate relaxation. Immunohistochemistry of aquaporins AQP1 and AQP4 showed that the water-channel proteins were overexpressed in invasion but not in proliferation, suggesting that relaxations at low-field are modulated by water-exchange under the AQP1 and AQP4 control. The method can be extended to FFC imaging.

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