Meeting Banner
Abstract #1064

Direct detection of 2HG and glutamate production using hyperpolarized [1-13C-5-12C]-a-ketoglutarate in cell and in vivo glioma models

Donghyun Hong1, Yaewon Kim1, Chandrasekhar Mushti2, Noriaki Minami1, Anne Marie Gillespie1, Pavithra Viswanath1, Rolf E. Swenson2, Daniel B. Vigneron 1, and Sabrina M. Ronen1
1University of California, San Francisco, San Francisco, CA, United States, 2NHLBI, Bethesda, MD, United States


Mutant IDH leads to 2HG production, which drives glioma development. 13C MRS monitoring of hyperpolarized [1-13C]α-ketoglutarate (αKG) metabolism to 2HG and glutamate provides a non-invasive assessment of the IDH mutation and normal metabolism, respectively. However, monitoring 2HG production in vivo is challenging because its resonance is within 0.1 ppm of the natural abundance [5-13C]αKG signal of the [1-13C]αKG substrate. Here, we utilized [1-13C-5-12C]αKG, which eliminated the [5-13C]αKG peak. This new approach, combined with an optimized sequence, made it possible to readily monitor the production of both 2HG and glutamate in a patient-derived glioma animal model and in normal brain.

This abstract and the presentation materials are available to members only; a login is required.

Join Here