Phase-cycled spectroscopic imaging (PCSI) method was implemented and validated for 31P PCSI imaging at 7T. The PCSI method uses a balanced steady-state free precession sequence with an ultra-low flip angle (<1°) to achieve sharp passband with 2.52-ms of TR, which enable to accelerate the acquisition. With prior knowledge of 31P spectra, it is feasible to acquire major 31P peaks by changing the frequency offset and non-uniform phase sweeping instead of acquiring full spectra uniformly. To investigate feasibility of the method, a multi-compartment KH2PO4 phantom with in vivo equivalent concentrations was prepared and 31P PCSI was compared with conventional FID-CSI.
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