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Abstract #1311

Perfusion system for studying dynamic metabolomics in rat brain slices exposed to oxygen and glucose deprivation using 1H and 31P NMR

Deborah Hill1, Alicja Molska1, Trygve Andreassen1, and Marius Widerøe1
1Department of Circulation and Medical Imaging, Norwegian University of Science and Technology (NTNU), Trondheim, Norway


We designed an NMR-compatible bioreactor that allows real-time metabolic measurements of viable rat brain slices under normal conditions and under conditions mimicking hypoxic-ischemic (HI) brain injury. Special emphasis was put on providing physiological temperature in the system, a significant factor in the HI model. 1H NMR spectra were acquired to assess changes in brain metabolites. Peaks of lactic acid, NAA, glutamate, aspartate, and creatine were detected. By acquiring a time series of proton spectra, we observed significantly increased lactate levels over time when switching from normoxia to hypoxia while other metabolites remained stable over the whole experiment.

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