Filter-exchange spectroscopy (FEXSY) and imaging (FEXI) are sensitive to transmembrane water exchange, which is commonly altered during cell death1. Yeast cells in different permeabilization stages were studied with FEXI, DWI and CFDA-AM/ propidium iodide (PI) staining using flow cytometry. Cell populations with 3.8% PI positive staining had 44% increased AXR, but unchanged ADC. At 33% nonvital cells, detection of AXR became unreliable and ADC increased. In vivo FEXI and DWI in EL4 lymphoma, with H&E-confirmed necrotic tumor cells and edema, showed AXR and ADC variations across the tumor, possibly due to differing vitality cell stages.
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