Abstract #1631

Filter-exchange spectroscopy and imaging sensitively detect necrotic cell death in vitro and in vivo

Athanasia Kaika¹, Geoffrey J. Topping¹, Simone Ballke², Thomas Theodor Metzler², Irina Heid³, Mathias Schillmaier¹, Rickmer Braren³, and Franz Schilling¹

¹Department of Nuclear Medicine, TUM School of Medicine, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany, ²Institute of Pathology, TUM School of Medicine, Technical University of Munich, Munich, Germany, ³Institute of Radiology, TUM School of Medicine, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany

Synopsis

Filter-exchange spectroscopy (FEXSY) and imaging (FEXI) are sensitive to transmembrane water exchange, which is commonly altered during cell death¹. Yeast cells in different permeabilization stages were studied with FEXI, DWI and CFDA-AM/ propidium iodide (PI) staining using flow cytometry. Cell populations with 3.8% PI positive staining had 44% increased AXR, but unchanged ADC. At 33% nonvital cells, detection of AXR became unreliable and ADC increased. In vivo FEXI and DWI in EL4 lymphoma, with H&E-confirmed necrotic tumor cells and edema, showed AXR and ADC variations across the tumor, possibly due to differing vitality cell stages.

This abstract and the presentation materials are available to members only; a login is required.

Join Here