Development of 13C MRS measurements of hepatic glutathione production: monitoring oxidative stress in vivo
Stephen Bawden1,2, Bernard Lanz3, Peter E Thelwall4, Guruprasad P Aithal2, and Penny Gowland1
1Sir Peter Mansfield Imaging Centre, School of Physics and Astronomy, University of Nottingham, Nottingham, United Kingdom, 2NIHR Nottingham Biomedical Research Centre, Nottingham University hospitals NHS trust and the University of Nottingham, University of Nottingham, Nottingham, United Kingdom, 3Sir Peter Mansfield Imaging Centre, School of Medicine, University of Nottingham, Nottingham, United Kingdom, 4Translational and Clinical Research Institute, Newcastle University, Newcastle, United Kingdom
Oxidative stress plays a central role in the development of both acute and chronic liver injury, with glutathione being the primary anti-oxidant. This study develops a method of measuring glycine to glutathione flux in vivo using 13C MRS and a novel labelling strategy. Quantification of [2-13C] glycine and [2-13C] glutathione concentrations following glycine ingestion show that the protocol used in this study does successfully enrich the glutathione pool and confirm the assumptions of the metabolic model proposed. This provides a powerful methodology to investigate oxidative stress in patient populations and in response to drug intervention.
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