Oxidative stress plays a central role in the development of both acute and chronic liver injury, with glutathione being the primary anti-oxidant. This study develops a method of measuring glycine to glutathione flux in vivo using 13C MRS and a novel labelling strategy. Quantification of [2-13C] glycine and [2-13C] glutathione concentrations following glycine ingestion show that the protocol used in this study does successfully enrich the glutathione pool and confirm the assumptions of the metabolic model proposed. This provides a powerful methodology to investigate oxidative stress in patient populations and in response to drug intervention.
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