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Abstract #2325

Animal models using C57BL/6J mice are at risk of data misinterpretation due to random occurrences of animals with high levels of brain glutamine

Ivan Tkac1, Raghavendra Rao2, and Megan Paulsen2
1Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United States, 2Department of Pediatrics, University of Minnesota, Minneapolis, MN, United States

Synopsis

Wild-type and transgenic mice derived from the C57BL/6J mouse strain are widely used in animal models of different human diseases. However, this mouse strain suffers from random appearances of sporadic congenital portosystemic shunts resulting in extremely high glutamine levels in the brain. In our recent study aimed at effects of maternal obesity on their offspring we found that in 9 out of 31 studied C57BL/6J mice had high levels of brain Gln. The purpose of this abstract is to highlight the awareness of this persisting problem in animal models based on C57BL/6J background strain.

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