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Abstract #2332

The effect of gadolinium contrast agent on T1 and T2 relaxation of brain in live and fixed zebrafish MRI

Noémie Hamilton1, Claire Allen2, and Steven Reynolds3
1Neuroscience Institute, University of Sheffield, Sheffield, United Kingdom, 2The Bateson Centre, University of Sheffield, Sheffield, United Kingdom, 3Medical School, University of Sheffield, Sheffield, United Kingdom

Synopsis

Zebrafish have become a ubiquitous animal model for studying a range of diseases and conditions. Typically, these studies are conducted in transparent juvenal fish where optical imaging techniques can be used. However, this is more difficult in adult zebrafish, restricting their use in longitudinal studies. Brain pathology in adult zebrafish can be imaged by preclinical MRI. Administering gadolinium based contrast agents (GBCA), which can also reduce scanning time, can highlight brain abnormalities such as lesions. T1/T2 values have been reported for gadolinium treated fixed/sacrificed zebrafish, however, this has not been reported for live zebrafish.

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