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Abstract #2425

DEXSY Reveals Water Exchange Coupled to Activity

Nathan H. Williamson1,2, Rea Ravin1, Teddy X. Cai1,3, Melanie Falgairolle4, Michael J. O'Donovan4, and Peter J. Basser1
1National Institute of Child Health and Human Development, Potomac, MD, United States, 2National Institute of General Medical Sciences, Potomac, MD, United States, 3Department of Clinical Neurosciences, Wellcome Centre for Integrative Neuroimaging, FMRIB, University of Oxford, Oxford, United Kingdom, 4National Institute of Neurological Disorders and Stroke, Potomac, MD, United States


Currently, no MRI method exists to non-invasively and absolutely measure cellular activity. One approach is to quantify steady-state transmembrane water exchange rates. Diffusion Exchange Spectroscopy (DEXSY) non-invasively and directly encodes for exchange of endogenous components. Using DEXSY-based methods implemented on a low-field, high-gradient MR system, we show in viable ex vivo neonatal mouse spinal cord samples that the rate at which water exchanges across cell membranes decreases drastically after perturbation known to induce persistent membrane depolarization. We also show that the exchange rate recovers to normal values after perturbations known to restore membrane potential.

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