Abstract #2427

Elucidating dynamic anaerobic metabolism in obligate anaerobes with living cells

Leo Cheng¹, Aidan Pavao², Brintha Girinathan², Johann Peltier³, Pamela Altamirano Silva⁴, Bruno Dupuy⁵, Isabella Muti¹, Craig Malloy⁶, and Lynn Bry²

¹Radiology and Pathology, Massachusetts General HospitalMassachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States, ²Massachusetts Host-Microbiome Center, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States, ³Institut Pasteur, Université de Paris, Paris, France, ⁴Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San Jose, Costa Rica, ⁵Laboratory of the Pathogenesis of Bacterial Anaerobes, Institut Pasteur, Université de Paris, Paris, France, ⁶Radiology, The University of Texas Southwestern Medical Center, Dallas, TX, United States

Synopsis

Anaerobic microbial metabolism drives critical functions within global ecosystems, host-microbiota interactions, and industrial applications, yet remains ill-defined. We resolved dynamic metabolism in living cells of the anaerobic pathogen Clostridioides difficile using High-Resolution Magic Angle Spinning (HRMAS) Nuclear Magnetic Resonance (NMR) spectroscopy to inform genome-scale predictions of cellular metabolism. Analyses leveraged the sensitivity of ¹³C NMR spectroscopy to simultaneously track cellular carbon and nitrogen flow from fermentable ¹³C and ¹⁵N-labeled substrates. We illustrate a versatile approach to elaborate complex anaerobic metabolism for clinical, scientific, and industrial applications.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.