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Abstract #2767

The over-expression of human hydrolase hMTH1 modulates metabolism and fat composition in mice exposed to high fat diet: a MRI and MRS study

Rossella Canese1, Gabriele De Luca2, Taljinder Singh1, Ambra Dell'Orso3, Egidio Iorio1, Mattea Chirico1, Maria Elena Pisanu1, Paola Fortini3, and Valeria SImonelli3
1Core Facilities, Istituto Superiore di Sanita', Rome, Italy, 2Oncology and Molecular Medicine Dept, Istituto Superiore di Sanita', Rome, Italy, 3Environmental and Health Dept, Istituto Superiore di Sanita', Rome, Italy

Synopsis

Oxidative stress is implicated in cancer, neurodegeneration and aging. hMTH1 is a hydrolase able to remove oxidized precursors from nucleotide’s pool, thus avoiding oxidative nucleic acids damage. Overexpression of hMTH1 in mice is protective against oxidative damage, neurodegeneration and prolongs life span. Our study showed that the overexpression of hMTH1 in mice fed with high fat diet (HFD), a dietary regimen linked to inflammation, is associated with increased brown interscapular fat (linked to protection from obesity) and with reduced perivesical fat volume (indicator of poor cardiovascular outcomes) up to four weeks. These effects seem to be reversed by prolonging HFD.

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