Blockade of lymphatic drainage to deep cervical lymph nodes impacts CSF flow and brain morphometry
Sunil Koundal1, Zachary Gursky1, Xinan Chen2, Hedok Lee1, Laura Santambrogio3, Jonathan Kipnis4, Allen Tannenbaum2, and Helene Benveniste1
1Anesthesiology, Yale University, New Haven, CT, United States, 2Department of Applied Mathematics and Statistics, Stony Brook University, Stony Brook, NY, United States, 3Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, United States, 4Department of Pathology and Immunology, Washington University, St. Louis, MO, United States
The altered CNS fluid flow dynamics and homeostasis mechanisms in response to blockade of lymphatic drainage to deep cervical lymph nodes (dcLN) is poorly understood. We used multi-modality MRI and computational fluid dynamics approach to study the same in rats with electrocauterized afferent lymphatic vessels of dcLN. The brain morphometry of Cauterized-dcLN rats showed localized volume expansion in Pons, Hippocampus and Corpus-callosum, while glymphatic speed maps showed hyperdynamic CSF flow in ambient and quadrigeminal cisterns. These results clearly show that the blockade of CSF drainage to dcLN alters CNS fluid homeostasis/flow dynamics, long-term may result in waste accumulation and neurodegeneration.
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