The purpose of this study is to find the parameters that provide the optimal trade-off in Signal-to-noise-ratio (SNR) and contrast-to-noise ratio (CNR) for 3D ihMT m-FFE (3D-ihMT) and to explore the feasibility of that for the segmentability of gray and white matters in the cervical spinal cord compared with 2D ihMT m-FFE (2D-ihMT). We recommend a Flip angle of 7 and TFE factor of 5 as an optimal trade-off between segmentability and artifact level, at least 16 nr repetitions are required for robust segmentation. The segmentability of 3D-ihMT could be increased to a level close to that of 2D-ihMT.
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