Non-invasive focused ultrasound (FUS) offered attractive advantages to modulate neuronal activity. The functional connectivity (FC) between different brain regions is a dynamic process during a period of examinations. We aimed to explore the longitudinal effect of FUS-neuromodulation on secondary visual cortex lateral area (V2L) by dynamic FC. We assessed k-means analysis and dynamic network analysis of dynamic FC of Pre-FUS, Sham, 35-min, 3-hr, and 3-day after FUS sonication at V2L. We found the instant effect of FUS-neuromodulation (35-min) and the recovery in a longitudinal follow-up of 3-day, suggesting the potential usefulness of FUS-neuromodulation.