Abstract #0166

Multi-Modal Protein-Engineered Theranostic Fibers: Drug encapsulation, Imaging, and enhanced 19F MRS

Dustin Britton¹, Jakub Legocki^1,2, Orlando Aristizabal³, Orin Mishkit³, Chengliang Liu¹, Sihan Jia¹, Paul Douglas Renfrew⁴, Richard Bonneau^4,5,6, Jin Kim Montclare^1,3,7,8, and Youssef Z Wadghiri³

¹Department of Chemical and Biomolecular Engineering, NYU Tandon, Brooklyn, NY, United States, ²New York University, Brooklyn, NY, United States, ³Department of Radiology, NYU Grossman School of Medicine, New York, NY, United States, ⁴Flatiron Institute - Simons Foundation, New York City, NY, United States, ⁵Computer Science Department, NYU Courant, New York City, NY, United States, ⁶Center for Genomics and Systems Biology, NYU, New York, NY, United States, ⁷Chemistry, NYU, New York, NY, United States, ⁸Department of Biomaterials, NYU College of Dentistry, New York, NY, United States

Synopsis

Keywords: Probes & Targets, Multimodal, theranostic, temperature probe, fluorine

Motivation: Theranostic materials allows for simultaneous therapeutic and diagnostic disease intervention.

Goal(s): We aimed to engineer a protein-based theranostic with multiple imaging modalities.

Approach: Using noncannonical amino acid incorporation of trifluoroleucine (TFL), we synthesize fluorinated coiled-coil, Q2_TFL, imageable by ¹H MRI and high-frequency ultrasound, and sensitive to ¹⁹F MRS.

Results: Q2_TFL demonstrates reduced signal contrast in ¹H MRI, echogenic signals under high-frequency ultrasound, and enhances sensitivity in linear ratiometric ¹⁹F MRS. This allows for thermoresponsiveness and potential protein conformation analysis. Q2_TFL serves as a promising platform for versatile and effective theranostic agents, bringing together therapeutic and diagnostic modalities in a compact and efficient manner.

Impact: Q2TFL, a fluorinated protein fiber, enables drug delivery and offers unique multimodal imaging. It acts as a temperature probe and protein structure monitor, paving the way for innovative theranostic biomaterials.

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