Abstract #2956

Identification of 9.4T MRI sequences for enhanced cellular visualisation of Multiple Sclerosis lesions

Elisabetta Giacomelli^1,2,3, Ilaria Callegari^1,2,3, Riccardo Galbusera^1,2,3, Erik Bahn⁴, Mario Ocampo-Pineda^1,2,3, Po-Jui Lu^1,2,3, Alessandro Cagol^1,2,3,5, Jochen Leupold⁶, Bibek Dhital^1,2,3, Matthias Weigel^1,2,3,7, Dominik von Elverfeldt⁶, Valerij G. Kiselev⁶, Christine Stadelmann⁴, and Cristina Granziera^1,2,3

¹Translational Imaging in Neurology (ThINk) Basel, Department of Biomedical Engineering, Faculty of Medicine, University Hospital Basel and University of Basel, Basel, Switzerland, ²Department of Neurology, University Hospital Basel, Basel, Switzerland, ³Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University Hospital Basel and University of Basel, Basel, Switzerland, ⁴Institute of Neuropathology, University Medical Center, Göttingen, Germany, ⁵Department of Health Sciences, University of Genova, Genova, Italy, ⁶Division of Medical Physics, Department of Diagnostic and Interventional Radiology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany, ⁷Division of Radiological Physics, Department of Radiology, University Hospital Basel, Basel, Switzerland

Synopsis

Keywords: Multiple Sclerosis, High-Field MRI, 9.4T MRI, Multi-Gradient-Echo, Multi-Spin-Echo, FLASH, RARE, Post-mortem, Histopathological images, Histochemical staining, Lesion features, Microglia

Motivation: Accurately analyzing cell presence in multiple sclerosis (MS) lesions via magnetic resonance imaging (MRI) cannot be achieved without histopathological validation.

Goal(s): Identification of biomarkers in MRI responsible for capturing microstructural alterations in brain tissues.

Approach: Investigation of relations in the ratio between lesion and normal-appearing white matter (NAWM) in voxel values of postmortem 9.4 T MRI scans and in cell density values of histochemical images of three MS lesions.

Results: We identified effective MRI sequences enhancing the contrast between the different features of the lesions and the NAWM and linked them to different cell type presence, as detected via quantitative histochemical analysis.

Impact: The establishment of a robust connection between MRI data and histochemical features will improve our understanding of the MS lesions development and its impact on the brain microstructure.

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