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Abstract #3469

Selective probe of intra- and extra-cellular diffusion with non-invasive MRI using non-uniform oscillating gradients

Ignacio Lembo Ferrari1,2, Manuel Avellaneda1,2, Ezequiel L. Saidman1,2, Francisco Divi1, and Gonzalo A. Alvarez1,2,3
1Instituto Balseiro, CNEA, Universidad Nacional de Cuyo, S. C. de Bariloche, Argentina, 2Centro Atómico Bariloche, CONICET, CNEA, S. C. de Bariloche, Argentina, 3Instituto de Nanociencia y Nanotecnologia, CNEA, CONICET, S. C. de Bariloche, Argentina

Synopsis

Keywords: Microstructure, Diffusion/other diffusion imaging techniques

Motivation: Overcoming MRI resolution limitations is crucial for early-diagnosis of cellular-level pathologies. Achieving non-invasive MRI-images with cellular-resolution promises deeper insights into tissue-microstructure for advancing medical diagnosis.

Goal(s): We aim to obtain cellular-resolution by characterizing molecular diffusion within intra- and extra-cellular compartments. We use Non-uniform Oscillating-Gradient Spin-Echo (NOGSE) signal-filtering potential, to address this intricate molecular diffusion problem.

Approach: We employ diffusion-theory and the NOGSE-sequence, to quantitatively characterize yeast-cell phantoms mimicking tissue-microstructure.

Results: We successfully characterize distinct diffusion-regimes within and outside cells. We introduce an innovative method to discriminate intra- and extra-cellular signals without complex models and analyses, allowing selective measurements of specific tissue-microstructure compartments.

Impact: We introduce a transformative approach to non-invasive imaging, allowing cellular-level resolution discriminating different tissue-microstructure features without model assumptions. It offers a tool to investigate intricate cellular structures. Harnessing this technology might benefit patient early-diagnosis of diseases, based on quantitative images.

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Keywords