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Abstract #0155

Effect of extracellular space preservation and partial delipidation on ex vivo brain MRI tissue contrast and microstructure

Jingting Yao1,2, Hansol Lee1,2, Kwok-Shing Chan1,2, Xiaotang Lu3, Wei Wang4, Zhuhao Wu4, Jerome L. Ackerman1,2, Hong-Hsi Lee1,2, and Susie Y. Huang1,2
1Massachusetts General Hospital, Charlestown, MA, United States, 2Harvard Medical School, Boston, MA, United States, 3Department of Molecular and Cellular Biology, Harvard University, Boston, MA, United States, 4Appel Alzheimer's Disease Research Institute, Weill Cornell Medicine, New York, NY, United States

Synopsis

Keywords: Microstructure, Ex-Vivo Applications, validation, tissue preparation, diffusion, T2, T2*, extracellular space preservation, tissue clearing

Motivation: Accurate tissue preservation is crucial for ex vivo MRI as it better reflects living tissue and allows for more reliable comparison with histopathology.

Goal(s): To evaluate the impact of different tissue preparation techniques, including extracellular space (ECS) preservation and partial delipidation, on MRI-derived microstructure measures.

Approach: dMRI on ECS-preserved and partially-cleared mouse brains was compared against conventionally fixed tissue imaging. DTI and standard model imaging with dot compartment metrics were evaluated.

Results: ECS-preserved brain had higher mean diffusivity and extracellular signal fraction and reduced dot compartment fraction. The partially cleared mouse brain showed increased diffusivity without affecting T2 and T2* values.

Impact: Incorporating advanced tissue preparation methods in ex vivo MRI studies will improve accuracy of histopathologic correlations and translational relevance to in vivo imaging interpretation.

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Keywords