Keywords: Microstructure, Ex-Vivo Applications, validation, tissue preparation, diffusion, T2, T2*, extracellular space preservation, tissue clearing
Motivation: Accurate tissue preservation is crucial for ex vivo MRI as it better reflects living tissue and allows for more reliable comparison with histopathology.
Goal(s): To evaluate the impact of different tissue preparation techniques, including extracellular space (ECS) preservation and partial delipidation, on MRI-derived microstructure measures.
Approach: dMRI on ECS-preserved and partially-cleared mouse brains was compared against conventionally fixed tissue imaging. DTI and standard model imaging with dot compartment metrics were evaluated.
Results: ECS-preserved brain had higher mean diffusivity and extracellular signal fraction and reduced dot compartment fraction. The partially cleared mouse brain showed increased diffusivity without affecting T2 and T2* values.
Impact: Incorporating advanced tissue preparation methods in ex vivo MRI studies will improve accuracy of histopathologic correlations and translational relevance to in vivo imaging interpretation.
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