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Abstract #0253

Visualization of Glymphatic Route on the Rat Cerebral Surface Using Direct Injection of 17O-Labeled Water and Proton MRI

Hiroyuki Kameda1,2,3, Takaaki Fujii4,5, Naoya Kinota4,5, Daisuke Kato4,5, Yoshitaka Bito4, Minghui Tang4, and Kohsuke Kudo1,3,4
1Department of Diagnostic and Interventional Radiology, Hokkaido University Hospital, Sapporo, Japan, 2Faculty of Dental Medicine, Department of Radiology, Hokkaido University, Sapporo, Japan, 3Global Center for Biomedical Science and Engineering, Faculty of Medicine, Hokkaido University, Sapporo, Japan, 4Department of Diagnostic Imaging, Graduate School of Medicine, Hokkaido University, Sapporo, Japan, 5Department of Dental Radiology, Hokkaido University Hospital, Sapporo, Japan

Synopsis

Keywords: Neurofluids, Neurofluids, Glymphatic hypothesis, SLYM

Motivation: Understanding water dynamics on the cerebral surface is crucial for advancing neurofluid research and exploring the glymphatic hypothesis and the subarachnoid lymphatic-like membrane (SLYM).

Goal(s): Detailed visualization of water dynamics on the surface of the brain using a water tracer.

Approach: 17O-labeled water was directly injected into the subarachnoid space (SAS) of the rat cerebrum to increase local concentration for detailed observation of water dynamics.

Results: The inner SAS group showed a higher concentration of 17O-labeled water, with vertical distribution patterns observed on the cortical surface, suggesting potential pathways for CSF flow into brain parenchyma.

Impact: Using the technique of local subarachnoid injection of 17O-labeled water and an optimiszd proton MRI method, we were the first to visualize CSF inflow pathways in the cerebral cortex as distinct signal changes.

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Keywords