Meeting Banner
Abstract #1005

Optogenetic stimulation of cell bodies versus axonal terminals yields remarkably comparable activity and functional connectivity in the brain

LiMing Hsu1, Domenic Cerri2, and Yen-Yu Ian Shih3
1Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States, 2Department of Radiology and BRIC, Chapel hill, NC, United States, 3Department of Neurology, Chapel Hill, NC, United States

Synopsis

Keywords: Task/Intervention Based fMRI, Preclinical, Neuroscience, fMRI, preclinical image analysis, brain, small animals

Motivation: Optogenetic techniques are commonly used to dissect neural pathways with presumed specificity for targeted projections.

Goal(s): To explore the effects of stimulating cell bodies versus a projection terminal in the ventral subiculum to nucleus accumbens shell pathway.

Approach: Optogenetic fMRI was used to compare the effects of stimulating vSUB cell bodies and their terminals in NAcSh, analyzing brain activity and connectivity patterns.

Results: Terminal stimulation induces brain activity and connectivity patterns similar to cell body stimulation, suggesting broader network engagement through antidromic activation.

Impact: The fMRI findings from this study challenge the general assumption that optogenetic stimulation at synaptic terminals primarily recruits feedforward activity in downstream brain regions, urging the neuromodulation community to exercise caution in interpreting data obtained with terminal optogenetic approach.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.

Keywords