Abstract #1012

Neural activity underpins dynamic BOLD signals: findings from simultaneous wide-field fluorescent calcium imaging and fMRI.

Francesca Mandino¹, Corey Horien², Xilin Shen¹, Xenophon Papademetris^1,3,4,5, Shella Keilholz⁶, Nan Xu^6,7,8, and Evelyn MR Lake^1,3,5

¹Radiology and Biomedical Imaging, Yale School of Medicine, New Haven, CT, United States, ²Psychiatry, University of Pennsylvania, Philadelphia, PA, United States, ³Biomedical Engineering, Yale School of Medicine, New Haven, CT, United States, ⁴Biomedical informatics and data science, Yale School of Medicine, New Haven, CT, United States, ⁵Wu Tsai Institute, Yale School of Medicine, New Haven, CT, United States, ⁶Biomedical Engineering, Emory University/Georgia Tech, Atlanta, GA, United States, ⁷Department of Bioengineering, University of Maryland, College Park, MD, United States, ⁸Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, United States

Synopsis

Keywords: Preclinical Image Analysis, Multimodal, dynamic functional connectivity

Motivation: BOLD-fMRI measures whole-brain activity but lacks specificity, and suffers from low SNR, making it challenging to capture reliable dynamic activity patterns.

Goal(s): We aim to further validate the detection of quasi-periodic patterns (QPPs), as measured by BOLD, using simultaneous wide-field fluorescent calcium imaging (WF-Ca²⁺) and fMRI.

Approach: Mice with GCaMP-labelled excitatory neurons were imaged under isoflurane. QPP detection was accomplished using either cortical WF-Ca²⁺ or BOLD-fMRI data at the dataset- and subject-level.

Results: Significant cross-modal agreement in QPP spatiotemporal structure was uncovered alongside some expected inter-subject variability. These finding help to affirm the neural origins of patterned cortical activity as detected by BOLD.

Impact: Quasi-periodic patterns (QPPs), measured via BOLD-fMRI, are recurring low-frequency coordinated waves of brain activity that appear in humans and model species. Here, we extend the characterization of the neural underpinnings of QPPs using simultaneous wide-field fluorescent calcium imaging and BOLD-fMRI.

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