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Abstract #1014

Simultaneous fMRI and fluorescence calcium imaging of brain dynamics under hypercapnia in mice

Irmak Gezginer1,2, Zhenyue Chen1,2,3, Yi Chen1,2, and Daniel Razansky1,2
1ETH Zurich, Zurich, Switzerland, 2University of Zurich, Zurich, Switzerland, 3Tongji University, Shanghai, China

Synopsis

Keywords: Functional Connectivity, Multimodal, Fluorescence Imaging

Motivation: Hypercapnia-induced changes in neurovascular coupling are not well understood.

Goal(s): Our goal was to capture and differentiate the neural and hemodynamic effects of hypercapnia.

Approach: We used concurrent fMRI and fluorescence calcium imaging in mice under elevated CO₂, analyzing spatiotemporal patterns of functional connectivity across modalities.

Results: Findings showed a distinct decoupling between neural and vascular responses under hypercapnia. Notably, BOLD signals did not transition into a neural state, remaining in a baseline vascular mode with significantly dampened fluorescence-triggered responses. In contrast, FL signals transitioned into the neural mode more frequently, revealing adaptive shifts in neural dynamics under hypercapnic stress.

Impact: By differentiating neural and vascular responses under hypercapnia, this study highlights limitations of BOLD as a neural marker during CO₂ stress. Findings inform strategies to restore neurovascular balance, relevant to chronic hypercapnic disorder diagnosis and treatment.

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