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Abstract #1813

Imaging aberrant oxidative and gluconeogenic metabolism in acute kidney injury using hyperpolarized [1-13C]pyruvate and [1,4-13C2]fumarate

Sasanka Nadishan Gunarathna Wathukara Dewage1, Sung-Han Lin2, Mai Huynh2, Zohreh Erfani2, Xiaodong Wen2, Zoltan Kovacs2, and Jae Mo Park3
1Department of Biomedical Engineering, University of Texas Southwestern Medical Center, Dallas, TX, United States, 2Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, United States, 3Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Dallas, TX, United States

Synopsis

Keywords: Biomarkers, Hyperpolarized MR (Non-Gas), Gluconeogenesis, Fumarase, PEPCK, PDH

Motivation: Clinical diagnosis and management of acute kidney injury rely on systemic blood biomarkers, lacking specificity and sensitivity of associated renal metabolism.

Goal(s): To examine changes of renal oxidative and gluconeogenic metabolism induced by acute kidney injury.

Approach: Hyperpolarized (HP) [1-13C]pyruvate and HP [1,4-13C2]fumarate were separately used to assess pyruvate dehydrogenase (PDH) and phosphoenolpyruvate carboxykinase (PEPCK) activities in rat kidneys with unilateral ischemia reperfusion injury.

Results: [13C]Bicarbonate produced from HP [1-13C]pyruvate and HP [1,4-13C2]fumarate decreased, suggesting suppressed PDH flux and gluconeogenic pathway, respectively.

Impact: The proposed imaging methods and biomarkers can be broadly applicable to other types of tissue injuries, as well as renal or hepatic diseases that affect metabolism.

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Keywords