Abstract #2516

Brain Metabolic Alteration in Two Mouse Stroke Models: a Multi-field Comparison via Chemical Exchange Saturation Transfer (CEST) MRI

Kexin Wang^1,2, Licheng Ju^2,3, Guanda Qiao⁴, Yajie Liang⁴, Yihan Wu^1,2, Chengyan Chu⁴, Joshua Rogers⁴, Yuguo Li^2,3, Suyi Cao^5,6, Valina L. Dawson^5,6,7,8, Ted M. Dawson^5,6,8,9, Piotr Walczak⁴, and Jiadi Xu^2,3

¹Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States, ²Kennedy Krieger Institute, Baltimore, MD, United States, ³Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University, Baltimore, MD, United States, ⁴Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States, ⁵Neuroregeneration and Stem Cell Programs, The Institute of Cell Engineering, Johns Hopkins University, Baltimore, MD, United States, ⁶Department of Neurology, Johns Hopkins University, Baltimore, MD, United States, ⁷Department of Physiology, Johns Hopkins University, Baltimore, MD, United States, ⁸Department of Neuroscience, Johns Hopkins University, Baltimore, MD, United States, ⁹Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, MD, United States

Synopsis

Keywords: Stroke, Preclinical, Methods, Metabolism, pH, neurotransmitter, Biomarkers, MCAO

Motivation: Monitoring metabolite and neurotransmitter changes resulting from stroke injuries is critical for diagnosis and treatment but remains challenging in vivo.

Goal(s): To demonstrate metabolic change difference between two stroke models via chemical exchange saturation transfer (CEST) MRI at both high-field and clinical scanners.

Approach: Mouse models of permanent middle cerebral artery occlusion (pMCAO) and transient MCAO (tMCAO) were scanned at 9.4T and 3T. Guanidino, amine, and amide CEST provided insights into metabolite, neurotransmitter, and pH changes, with MRS and simulations validating findings.

Results: Creatine levels increase significantly in pMCAO with larger pH drop, compared to tMCAO, which shows a notable increase in amineCEST.

Impact: Simultaneous monitoring of brain metabolites, neurotransmitters, and pH levels will advance understanding and treatment of ischemic stroke and other metabolic brain diseases, positioning chemical exchange saturation transfer (CEST) MRI as a promising tool.

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